ABMI

Incubation / Evaluation


 

Storage Conditions

For low acid products

  • In case of culture medium or clear product in clear bottles, all 30,000 bottles are visually inspected after 7 days of incubation at a temperature of 30°-32°C.
  • In case of turbid product in clear bottles, all 30,000 bottles are visually inspected after 7 days of incubation at a temperature of 30°-32°C. Additionally 1,000 bottles per run are checked for pH.
  • In case of non-transparent bottles, 30,000 bottles are visually inspected after 7 days of incubation at a temperature of 30°-32°C. Defective bottles change their size or their shape (gas production or vacuum generation). Additionally 3,000 bottles per run are checked for pH level.

 

For high acid products

  • Half filled or completely filled bottles are stacked on pallets and stored in a secured place at constant temperature of 25 to 30°C for 14 days. All 90,000 bottles are visually inspected.
  • Half filled bottles with liquid nitrogen treatment are stored at constant temperature of 25 to 30°C for 14 days. All 90,000 bottles are visually inspected.
  • Completely filled bottles with liquid nitrogen treatment are stored at constant temperature of 25 to 30°C for 21 days. All 90,000 bottles are visually inspected.

Inspection

After storage, all the bottles are visually inspected.Only hermetically sealed packages are taken into consideration where the integrity has been assured during all the production steps.

Evaluation

All packages failing microbiological testing are to be saved for package integrity testing. If any contaminated bottle is found, microbiological identification is carried out in order to determine the origin of the contamination. Especially heat resistant spore identification must be carried out in order to exclude microorganisms coming from the raw material that may have survived the thermal processing.