9. Fill Level of the Bottles

Half fill the bottles in order to shorten the incubation time because of a higher oxygen level in the headspace.
If bottle handling (transport, stacking) is critical, the fill level may be increased up to the necessary level e.g. 80% of the nominal volume.
If nitrogen is used in commercial production, the validation should also include nitrogen dosing.
If the customer wants to have sellable product, the bottles may be filled with product up to the nominal volume.